Creative Biolabs utilizes state-of-the-art synthesis platforms, primarily employing solid-phase synthesis to produce high-quality oligonucleotides. This method involves tethering the oligonucleotide to a solid support material throughout the synthesis process, ensuring efficient and controlled chain assembly.The solid-phase synthesis process begins with the 3'-most nucleotide and proceeds through a series of cycles, each consisting of four key steps:
- Deprotection: Removal of protecting groups to enable the next nucleotide addition.
- Condensation: Coupling of the next nucleotide to the growing oligonucleotide chain.
- Capping: Blocking unreacted 5'-OH groups to prevent unwanted side reactions.
- Oxidation: Conversion of the phosphite linkage to a stable phosphate linkage.
This cyclical process, based on the phosphoramidite approach, is repeated until the 5'-most nucleotide is attached, resulting in the complete oligonucleotide sequence.
Creative Biolabs offers a wide range of oligonucleotide synthesis services to meet diverse research needs. They specialize in synthesizing complex oligonucleotides, including:
- MicroRNAs (miRNA): Small non-coding RNA molecules involved in gene regulation.
- Small-interfering RNAs (siRNAs): Double-stranded RNA molecules used for RNA interference.
- Messenger RNAs (mRNA): RNA molecules that carry genetic information from DNA to ribosomes for protein synthesis.
- Bridged Nucleic Acids (BNA): Modified nucleic acids with enhanced binding affinity and stability.
In addition to these standard oligonucleotides, Creative Biolabs provides custom synthesis services to create oligonucleotides tailored to your specific requirements. They offer personalized solutions based on your unique oligonucleotide modifications, ensuring optimal performance in your research applications.
Key Advantages of Partnering with Creative Biolabs
- High-Quality Custom-Manufactured Oligonucleotides: Creative Biolabs is committed to producing oligonucleotides of the highest quality, ensuring accurate sequences and consistent performance.
- Complete Quality Control Package: They provide a comprehensive quality control package, including purity analysis and mass spectral analysis, to verify the integrity and accuracy of the synthesized oligonucleotides.
- One-Stop Synthesis Services: Creative Biolabs offers a complete suite of synthesis services, streamlining your project and reducing the overall timeline.
- Experienced Staff and Advanced Technologies: Our team of experienced scientists utilizes advanced technologies and techniques to deliver exceptional results.
Your Trusted Partner for Oligonucleotide Synthesis- Contact Creative Biolabs today to discuss your oligonucleotide synthesis needs and experience the difference.